Figure 8

iNOS inhibition increases dendritic cell migration and increases lymphatic pumping frequency.

(a) Representative flow cytometry plots of CD11c⁺/MHC-II⁺/CD45.1⁺ transplanted dendritic cells harvested from popliteal lymph nodes 18 hours after injection in the distal hindlimb. Red box indicates gating used to determine CD11c⁺/MHC-II⁺ double-positive dendritic cells. (b) Quantification of CD11c⁺/MHC-II⁺/CD45.1⁺ transplanted dendritic cells (n = 5 animals/group, p < 0.01). (c) Representative whole mount images of mouse ears stained for iNOS (green), CD11b (red) and Podoplanin (white). Note decreased iNOS⁺ cell accumulation in obese mice treated with 1400W. (d) Quantification of perilymphatic iNOS⁺/CD11b⁺ cells in whole mount stained tissues (n = 4 animals/group, p < 0.05). (e) Representative line graphs showing lymphatic contractions over time. Peaks in intensity correspond with lymphatic contractions and are shown as arbitrary units. (f) Quantification of lymphatic contractions (lymphatic contractions per minute; n = 5 animals per group, p < 0.05).