Figure 2
From: Tcstv1 and Tcstv3 elongate telomeres of mouse ES cells
Tcstv1 and Tcstv3 extend telomere lengths in mouse ESCs.
(a) Representative telomere Q-FISH images of Tcstv1 OE, Tcstv3 OE and mock ESCs at P10. Telomeres are labeled with telomere PNA probes (green) and chromosomes are labeled with DAPI (blue). Scale bar = 10 μm. (b) Histogram shows distribution of relative telomere length expressed as fluorescence intensity (TFU, telomere fluorescence unit) by telomere Q-FISH analysis. Green line is median telomere length. Average telomere length is shown as mean TFU ± SD. P value, compared to mock ESCs. (c) Telomere restriction fragment (TRF) analysis showed increased telomere lengths in Tcstv1 OE and Tcstv3 OE ESCs compared with mock ESCs at P9.
