Figure 1 | Scientific Reports

Figure 1

From: Characterization of the GntR family regulator HpaR1 of the crucifer black rot pathogen Xanthomonas campestris pathovar campestris

Figure 1

HpaR1 positively regulates xanthan gum production in Xcc.

(A) The hpaR1 mutant produced significantly less xanthan gum, compared to the wild-type strain. Mean weight of xanthum gum extracted from the HpaR1 mutant strain 2736nk, the wild type strain 8004 and the complemented strain C2736. (B) The expression of gumB is affected positively by HpaR1 in xanthum producing medium, as revealed by promoter-reporter analysis. The graph shows the mean ± standard deviation (n = 3) gumB promoter-GUS activity in the wild type strain 8004 compared to the hpaR1 mutant strain 2736nk. The experiment was repeated twice and similar results were obtained. (C) The expression of gumB is positively regulated by HpaR1, as revealed by qRT-PCR analysis of the transcription of gumB in Xcc wild type and hpaR1 mutant strains. Values given are the means ± standard deviations of triplicate measurements from a representative experiment; similar results were obtained in two other independent experiments.

Back to article page