Figure 4 | Scientific Reports

Figure 4

From: Characterization of the GntR family regulator HpaR1 of the crucifer black rot pathogen Xanthomonas campestris pathovar campestris

Figure 4

HpaR1 interacts directly with the gumB promoter, as revealed by an electrophoretic mobility shift assay.

A FAM-labelled 529-bp DNA fragment of the gumB promoter (A) and 311-bp DNA fragment of the promoter of XC_1045 (as a control) (B) were incubated with purified His6-HpaR1 protein for 20 min at 30 °C, respectively. Lanes 1–5, FAM-labelled DNA (1.0 nM) with 0, 5, 10, 15 and 20 nM of His6-HpaR1 protein, respectively.

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