Figure 6
Restoring TGF-β2 expression reverses the regulation of miR-193b-3p on Trophoblast (HTR-8/SVneo) cells migration and invasion.
Simultaneous transfection of HTR8/SVneo cells with the miR-193b-3p inhibitor and TGF-β2 siRNA reduced the ability of the miR-193b-3p inhibitor to upregulate TGF-β2 mRNA expression at 48 h after transfection and co-transfection of pcDNA-TGFβ2 restored TGF-β2 expression (A). Overexpression of TGF-β2 (pcDNA-TGFβ2) attenuates the functions of miR-193b-3p to inhibit cell migration and cell invasion at 48 h after transfection (B). Co-transfection of TGF-β2 siRNA reduced the ability of miR-193b-3p inhibitor to promote cell migration at 48 h after transfection (C). Co-transfection of TGF-β2 siRNA abolished the increased cell invasion promoted by transfection of the miR-193b-3p inhibitor at 48 h (C).
