Figure 1

Lipid droplets numbers are modulated in response to Gram-negative bacteria and virus infection in Aag2 cells.

(A) Differential interference contrast (DIC) or (B) BODIPY 493/503 staining for LDs visualization (green) of Aag2 resting cells. Nuclei were stained with DAPI (blue). (C) Resting cells were stained with osmium tetroxide (OsO₄) and LDs can be visualized as black dots in the cytoplasm. (D) Resting Aag2 cells or (E) cells incubated for 24 hours with heat-killed gram negative bacteria Enterobacter cloacae, were stained with BODIPY 493/503 and DAPI. (F) Resting Aag2 cells (C - Control) or cells incubated for 24 hours with heat killed gram positive (G + , Micrococcus luteus), gram negative (G−, Enterobacter cloacae) bacteria, zymosan (Zy) or 20 μM heme (Hm) were stained with osmium tetroxide and the number of LDs per cell following different types of stimuli were counted on an optical microscope and represented in the graph. (G,H) Aag2 cells mock infected (G) or infected with Dengue 2 virus (H) were stained with BODIPY 493/503 and DAPI for LDs visualization. (I) Aag2 cells were infected with Dengue 2 for 7 days or Sindbis virus for 4 days and stained with osmium tetroxide for LD counting. Figure represents the percentage distribution of the numbers of LDs per cell. In (F) for statistical analyses, we used ANOVA followed by Dunnett’s multiple comparison test.