Figure 2

Several Aedes aegypti genes are modulated by infections with both gram negative bacteria and virus.

Aag2 cells were exposed to either heat-killed Enterobacter cloacae (Gram negative, Ec) for 6 hours or to Dengue virus (Halstead strain) for 4 days. After these periods total RNA was used in a microarray analysis aiming to identify genes regulated in both conditions. (A) Venn diagram showing genes modulated by bacterial infection (pink circle, left) viral infection (blue circle, right) or modulated by both infections (intersection of the circles). The green arrowhead indicates the number of up-regulated genes and the red arrowhead the number of down-regulated genes in each condition. (B) Functional classification of the genes modulated by bacterial or viral infection. The graph shows the functional class distribution of modulated genes, obtained using gene ontology software. (http://www.geneontology.org/). See Dataset S1 for complete gene expression changes dataset.