Figure 3
From: Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
Bacterial and viral infections lead to an increase in the number of LDs in mosquito midgut.
Aedes aegypti females were fed with a saline solution containing live Serratia marcescens and 24 hours after infection, midguts were stained with Nile Red for visualization of LD numbers. (A,C) Differential interference contrast (DIC); (B,D) Nile red stained midguts (red). Alternatively, Aedes aegypti females were fed with Sindbis virus and 4 days post infection, midguts were stained with Nile Red. (E) Mock infected mosquitoes; (F) Sindbis infected mosquitoes. Nuclei were stained with DAPI (blue). (G,H) Fluorescent particle quantification was performed using Analyze particles tool from ImageJ.
