Figure 3

Western blot analysis of endogenous hBACE1 and β-actin levels in the cell lysate of N2a695 cells treated with different concentrations of pre-miR-29b, at 24, 48 and 72 h.

(A–C): representative images of Western blot from N2a695 cells after 72 h transfection with CS/pre-miR-29b, PEI/pre-miR-29b and Synthetic miR-29b and Scrambled RNA, respectively. (D–F): densitometric quantifications of hBACE1 expression from Western blot images of CS/pre-miR-29b, PEI/pre-miR-29b and Synthetic miR-29b and Scrambled RNA, respectively. Error bars represent standard deviations derived from three or more independent experiments performed in duplicate. ANOVA, mean ± SD. (For simplicity purposes, the blots were cropped).