Figure 2
Insulin induced C-Fos expression in PVH.
Wild-type mice were icv administered with saline, NPY (2 μg in 2 μl) and/or insulin (10mU in 2 μl). C-Fos expression in the PVH was examined 2 hours later. (a) Representative pictures showing C-Fos immunoreactive neurons in the PVH of NPY-treated mice. (b) C-Fos immunoreactive neurons in the PVH of insulin-treated mice. (c) C-Fos immunoreactive neurons in the PVH of insulin- and NPY-treated mice. (d) Statistical analysis of the number of C-Fos neurons in the PVH of mice described in (a–c). Scale bar is 500 μm. 3 V: the third ventricle. Data presented as mean ± SEM, n = 5–6 mice, **p < 0.01 vs saline, two-tailed unpaired student’s t-test.
