Figure 3
Fasting failed to induce C-Fos in PVH.
Wild-type mice were well-fed or fasted overnight and C-Fos expression in the PVH was examined at the end of fasting. (a) Representative pictures showing C-Fos immunoreactive neurons in the PVH of non-fasted mice. (b) C-Fos immunoreactive neurons in the PVH of fasted mice. (c) Statistical analysis of the number of C-Fos neurons in the PVH of mice described in (a,b). 3 V: the third ventricle. Scale bar is 500 μm. Data presented as mean ± SEM, n = 5–6 mice, two-tailed unpaired student’s t-test.
