Figure 4

Fasting-refeeding induced C-Fos in PVH.

STZ-treated (150 mg/kg) insulin deficient mice and controls were well-fed or 2 hour-refed after overnight fasting and C-Fos expression in the PVH was examined after refeeding. (a) Serum insulin concentration in 4 groups after refed 15 min. (b) Food intake in 4 groups within the 2-hour testing period. (c) Representative pictures showing C-Fos immunoreactive neurons in the PVH of control (upper panels), STZ mice (bottom panels), non-fasting (left panels) and fasting-refeeding treatment (right panels). (d) Statistical analysis of the number of C-Fos neurons in the PVH of mice described in (c). Ctrl N-F: Control non-fasting mice, Ctrl F-R: Control fasting-refeeding mice, STZ N-F: STZ non-fasting mice, STZ F-R: STZ fasting-refeeding mice. Scale bar is 500 μm. 3 V: the third ventricle. Data presented as mean ± SEM, n = 5–6, **p < 0.01, two way ANOVA tests with tukey post hoc analyses.