Figure 5
NPY induced C-Fos expression in PVH independent of GABAergic action on PVH neurons.
Sim1-Cre:γ2flox/flox KO mice and control mice were icv administered with saline or NPY (2 μg in 2 μl) and C-Fos expression in the PVH was examined 2 hours later. (a) Representative pictures showing C-Fos immunoreactive neurons in the PVH of WT (control, upper panels), KO mice (Sim1-Cre:γ2flox/flox, bottom panels), saline treatment (left panels) and NPY treatment (right panels). (b) Statistical analysis of the number of C-Fos neurons in the PVH of mice described in (a). Scale bar is 500 μm. 3 V: the third ventricle. Data presented as mean ± SEM, n = 5–6 mice, *p < 0.05 and **p < 0.01, two way ANOVA tests with tukey post hoc analyses.
