Figure 3 | Scientific Reports

Figure 3

From: The solution configurations of inactive and activated DntR have implications for the sliding dimer mechanism of LysR transcription factors

Figure 3

Crystal packing and SAXS analysis of the solution conformations ofapo-DntR, H169T-DntR and holo-DntR.

(a) An examination of the packing of the crystal form obtained for both apo- and H169T-DntR shows that this constrains apo- and H169T-DntR to adopt the same compact tetrameric configuration. Here anapo-DntR tetramer is shown in red and yellow and symmetry related DntR monomers are shown in green. For clarity not all symmetry-relate monomers in close contact with the tetramer are shown. (b) Experimental solution scattering curves (open squares) and the resulting fitted model (solid line) for apo-DntR (orange), H169T-DntR (green) and holo-DntR (blue). (c) Guinier plot regions the fits of which (solid lines, colour scheme as in (b)) yield the radii of gyration (Rg) shown. (d) The pair distribution functions P(r) indicating Dmax for apo-DntR (118.2 Å), H169T-DntR (135 Å) holo-DntR (142 Å). Colour scheme as in (b). (e) Kratky plots (colour scheme as in (b)) for apo-, H169T- andholo-DntR.

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