Figure 1
miR-203 is down-regulated in LSCs and its over-expression inhibits proliferation and stemness in vitro.
(A) qRT-PCR analysis of miR-203 expression was conducted on 15 peripheral blood samples from healthy volunteers and 50 paired CD34 + /CD34− cells from the blood samples of AML patients. (B) miR-203 expression was detected in LSC-enriched KG-1a and MOLM-13 cell lines and referenced against RNU6B. (C) Viability of KG-1a-LSCs and MOLM13-LSCs was analyzed following transfection with miR-NC or miR-203 with or without Ara-C. (D) In KG-1a-LSCs, the percentage of the CD34 + CD38− cells (upper left quadrant) in miR-NC and miR-203-transfected cells was detected by flow cytometry. (E) LSC cells transfected with miR-203 or miR-NC (48 h) were seeded in 6-well plates in soft agar at clonal density and cultured for 2 weeks. Cell colonies were then stained with Giemsa and photographed. (F) Representative photographs showing the spheroids formed by miR-203 or miR-NC expression in LSC cells. All data represent the mean ± SD from three independent experiments in each condition (*P < 0.05, **P < 0.01).
