Figure 2
Survivin is a direct target of miR-203.
(A) Venn diagram showing overlap in miR-203 targets predicted by three miR- research databases. The overlap contains 24 candidate genes that need further confirmation. (B) miR-203 directly targets the survivin 3′-UTR and is highly evolutionary conserved in different species (Has, Ptr, Mml, Fca, Eca and Bta). (C) Survivin mRNA and (D) protein levels in miR-NC and miR-203 groups, analyzed by qRT-PCR and Western blot 48 h after transfection. GAPDH was used as an internal control (*P < 0.05). (E) Schematic diagram of the survivin3′-UTR pMIR-REPORT constructs and alignment of between mature wild-type (WT) and mutated (Mut) miR-203 putative target sites in the 3′-UTR of survivin. (F) LSC cells were co-transfected with pMIR-REPORT constructs containing empty (Ctrl), wild-type, or mutant target site of the survivin 3′-UTR plus miR-203 or miR-NC. Luciferase activity was normalized to Renilla activity and presented as relative to miR-NC (*P < 0.05). All graphs depict are representative of at least three independent experiments (mean ± SD).
