Figure 3 | Scientific Reports

Figure 3

From: miR-203 inhibits proliferation and self-renewal of leukemia stem cells by targeting survivin and Bmi-1

Figure 3

miR-203 inhibited proliferation and prompted apoptosis in LSCs.

(A) Analysis of survivin mRNA and protein expression in KG-1a and MOLM13-LSCs 48 h after transfection with survivin-siRNA. Non-targeting (NT)-siRNA was used as a negative control (*P < 0.05). (B) The viability of LSCs was detected from 12 h to 48 h after transfection with survivin-siRNA or miR-203. (C) Analysis of survivin protein expression and (D) cell viability in LSCs following transfection with miR-203 and a survivin vector lacking the survivin 3′-UTR. (E) Analysis of apoptosis rates in KG-1a-LSCs 48 h after transfection with miR-203, survivin-siRNA and survivin transgene devoid of its 3′-UTR. miR-NC and siRNA-NC were used as negative controls (*P < 0.05, **P < 0.01).

Back to article page