Figure 1
From: CRISPRi-Manipulation of Genetic Code Expansion via RF1 for Reassignment of Amber Codon in Bacteria
Schematic representative of assignment of amber codon as either a stop or encoding signal in genetic code expansion.
(A) Schematic representative of the different translation routes resulting from the competition between RF1 and suppressor tRNA with the amber codon that leads to the truncated and read-through proteins as the respective products. (B) Schematic representative of the sgRNA-guiding, CRISPRi-mediated repression of RF1 for reassignment of amber from a stop codon to a sense codon.
