Figure 1
From: Kallikrein-related peptidase 8 is expressed in myocardium and induces cardiac hypertrophy
KLK8 is upregulated in hypertrophic hearts.
(A) Expression of KLK8 in myocardium. The left panel showed negative control. Pre-absorption of the KLK8 primary antibody was performed with a 10-fold excess of the blocking peptide. The right panel is the representative immunostained section of rat left ventricular myocardium with antibody against KLK8. The positive staining was mainly localized to cardiomyocytes as indicated by arrows. Original magnification, ×400. Scale bars correspond to 100 μm. (B,C) showed levels of cardiac hypertrophic markers (HW/BW, ANP, BNP and Myh7) and KLK8 in myocardium of Sham and TAC rats, respectively. D and E showed levels of cardiac hypertrophic markers (ANP, BNP and Myh7) and KLK8 in myocardium of WKY and SHR rats, respectively. F and G showed KLK1 levels in myocardium of Sham/TAC and WKY/SHR rats, respectively. Data were expressed as mean percentage of Sham or WKY ± SEM (n = 7). *P < 0.05, **P < 0.01 vs Sham. #P < 0.05, ##P < 0.01 vs WKY. Representative Western blotting bands are on the top of corresponding graphs.
