Figure 2
From: Effects of Chronic Low-Dose Radiation on Human Neural Progenitor Cells
Radiation dose-dependent DNA damage and cell loss in hNPCs.
hNPCs were exposed to radiation at doses of 31, 124 and 496 mGy for 72 h and the DNA damage responses were investigated. (a) Nuclei, neurons and DNA double-strand breaks were visualized by staining with Hoechst, anti-MAP2 and anti-γ-H2AX antibodies, respectively. White arrows indicate γ-H2AX foci in nuclei. Photographs obtained at 60 × magnification. (b) The number of nuclear γ-H2AX foci per cell was counted with a confocal microscope using 7–13 biological replicates. (c) The number of cells per field was also counted with a confocal microscope. (d) Cell cycle analysis after 72 h of irradiation with up to 496 mGy. hNPCs were stained with PI solution and analyzed with a FACS Caliber flow cytometer. (b,c) The graph data are the mean ± SD from seven to twelve biological replicates. *p < 0.05, ***p < 0.005 and ****p < 0.0001 by the Student’s t-test.
