Figure 7

The effects of XFZY on the PI3K-AKT-mTOR signaling pathway after TBI.

(A,B) The levels of p-AKT decreased significantly on the 1^st and 3^rd days in ipsilateral brain tissue after TBI. Treatment with 9 g/kg XFZY significantly reduced the increase in p-AKT expression from the 1^st to the 14^th day compared with the Vehicle group. The same trend was observed in the 18 g/kg XFZY group on the 1^st, 3^rd and 14^th days. The expression levels of p-AKT were significantly lower in the 9 g/kg XFZY group compared with the 18 g/kg XFZY group on the 7^th and 14^th days. No significant changes were detected in total AKT expression between the Sham and Vehicle group. (C,D) p-mTOR was significantly increased from the 1^st to the 14^th day after TBI. Treatment with 9 g/kg XFZY significantly decreased p-mTOR expression compared with the Vehicle group from the 1^st to the 14^th day. Treatment with 18 g/kg XFZY significantly reduced p-mTOR on the 1^st and 3^rd days. mTOR expression in the 9 g/kg XFZY group was significantly reduced compared with the 18 g/kg XFZY group on the 1^st, 7^th and 14^th days. There were no significant changes in total mTOR in comparisons between each group. (E,F) p-p70S6K increased significantly from the 1^st to the 14^th day after TBI. Treatment with 9 g/kg XFZY significantly reduced p-p70S6K expression on the above days. The same tendency was observed in the 18 g/kg XFZY group on the 1^st day. p-p70S6K expression was significantly reduced in the 9 g/kg XFZY group compared with the 18 g/kg XFZY group on the 3^rd, 7^th and 14^th days. No significant changes were detected in total p70S6K expression between the Sham and Vehicle group. Data are the mean ratio between targeting proteins and β-actin and are presented as the percentage of XFZY (or Vehicle)-treated brains over the control Sham-treated brains. The data represent the mean fold induction ± SEM, as analyzed by ANOVA. *p < 0.05, ^#p < 0.01 vs. the Vehicle group. ^∆p < 0.05, ^□p < 0.01 vs. the 9 g/kg XFZY group.