Figure 2
IL-10 production by transitional B-cells regulates T-cell responses indirectly via down-regulation of CD86.
(A) Dot plots and total percentages of T-cell proliferation and TNF-α production of anti-CD3 activated CD4+T-cells co-cultured with B-cell subsets were measured in the presence of a neutralising anti-IL-10 receptor antibody (0.1 μg/ml) or isotype control after 72 h. Bars in graphs represent the mean and standard error of 4 different experiments ***P < 0.001 and *P < 0.05 by Repeated Measures Two-way ANOVA test with Sidak’s multiple comparison test. (B) CD4+T-cell proliferation, TNF-α levels, IL-2 levels and IL-10 levels in culture were measured in isolated CD4+T-cells (1 × 105) activated with anti-CD3/CD28 in the presence of different concentrations of exogenous IL-10 by surface staining and CBA. Bars in graphs represent the mean and standard error of 3 different experiments **P < 0.01 by Kruskal-Wallis test with Dunn’s multiple comparison test. (C) Histograms and total percentages of T-cell proliferation of 1 × 105 anti-CD3 activated CD4+T-cells co-cultured with 1 × 105 B-cell subsets was measured in the presence of CD86-expressing CHO-cells and control CHO-cells (5 × 103) after 72 h. Bars in graphs represent the mean and standard error of mean of 3 different experiments *P < 0.05 by Repeated Measures Two-way ANOVA followed by Sidak’s multiple comparison test.
