Figure 4

Comparison of mRNA levels of key myogenic regulatory factors between control and dysp skeletal muscle.

Displayed are mRNA levels of the regulatory factors Six1, Six4. Pax3, Pax7, Myf5, MyoD, MyoG and Mrf4, as determined by microarray analyses and via qRT-PCR. Four biological replicates (4x control, 4x dysp; 8 animals in total) were run for every gene. In the qRT-PCR analyses, the respective Gapdh mRNA levels served as endogenous reference. The values on the ordinate emanate from normalizing the FCs of the 4 biological replicates (control or dysp) to the mean of the respective control. Thus, all controls amount to a “Fold change” of 1. Unpaired t-tests were performed for control vs. dysp for each gene, *represents a P value ≤ 0.05, **represents a P value ≤ 0.01 and ***represents a P value ≤ 0.001. Error bars are S.E.M.