Figure 4

TNF-α and IL-1β or activated p65 promotes YY1 expression and translocation.
(A) HEK293T cells were transfected with pCMV-p65 and the expression levels of p65 and YY1 were detected by Western blotting. (B) RA FLSs were stimulated with TNF-α or IL-1β, either alone or in combination with Bay 11–7082 for 24 h and the YY1 level was detected by Western blotting. The data are representative of three experiments (Supplementary Fig. S3).