Figure 6 | Scientific Reports

Figure 6

From: A novel NF-κB/YY1/microRNA-10a regulatory circuit in fibroblast-like synoviocytes regulates inflammation in rheumatoid arthritis

Figure 6

miR-10a repression promotes NF-κB activation and secretion of downstream inflammatory cytokines in FLS cells.

(A) FLS cells were stimulated as indicated and an immunofluorescence assay was performed 24 h later to detect the activation and translocation of NF-κB p65. (B) FLS cells (n = 3 samples, same as in Fig. 6C) were treated as indicated for 24 h and the mRNA levels of inflammatory cytokines or MMPs were measured by qRT-PCR. (C) Cells were treated as indicated and IL-6 and IL-8 production was determined by ELISA. nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group or between the indicated groups. The data are representative of three experiments.

Back to article page