Table 1 Nucleotide sequences of primers and siRNAs
Gene or product | Primers |
|---|---|
β-actin | Forward: 5′- CTGTCCACCTTCCAGCAGATGT-3′ |
Reverse: 5′- CGCAACTAAGTCATAGTCCGCC-3′ | |
YY1 (NM_003403) | Forward: 5′- AGAAGAGCGGCAAGAAGAGTT-3′ |
Reverse: 5′- CAACCACTGTCTCATGGTCAATA-3′ | |
IRAK-4 (NM_001145258) | Forward: 5′-CCTGACTCCTCAAGTCCAGAA-3′ |
Reverse: 5′-ACAGAAATGGGTCGTTCATCAAA-3′ | |
TAK1 (NM_003188) | Forward: 5′-AGCCTGATGACTCGTTGTTG-3′ |
Reverse: 5′-TAATGGCTCATCTGCTCCTG-3′ | |
BTRC (NM_003939) | Forward: 5′-CCAGACTCTGCTTAAACCAAGAA-3′ |
Reverse: 5′- GGGCACAATCATACTGGAAGTG -3′ | |
MCP-1 (NM_002982) | Forward: 5′-CAGCCAGATGCAATCAATGCC-3′ |
Reverse: 5′- TGGAATCCTGAACCCACTTCT -3′ | |
IL-6 (NM_000600) | Forward: 5′-CCCTGAGAAAGGAGACATGTAAC-3′ |
Reverse: 5′-CCTCTTTGCTGCTTTCACACATG-3′ | |
IL-8 (NM_000584) | Forward: 5′-TTGGCAGCCTTCCTGATTTC-3′ |
Reverse: 5′-TGGCAAAACTGCACCTTCAC-3′ | |
IL-1β (NM_000576) | Forward: 5′- TTCGACACATGGGATAACGAGG-3′ |
Reverse: 5′- TTTTTGCTGTGAGTCCCGGAG-3′ | |
MMP-1 (NM_002421) | Forward: 5′-GCTAACAAATACTGGAGGTATGATG-3′ |
Reverse: 5′-ATTTTGGGATAACCTGGATCCATAG-3′ | |
MMP-13 (NM_002427) | Forward: 5′- TCCTGATGTGGGTGAATACAATG -3′ |
Reverse: 5′- GCCATCGTGAAGTCTGGTAAAAT -3′ | |
siYY1 | Sense: 5′-CAGUCAACUAACCUGAAAUTT-3′ |
Antisense: 5′-AUUUCAGGUUAGUUGACUGTT-3′ | |
siNC | Sense: 5′-UUCUCCGAACGUGUCACGUTT-3′ |
Antisense: 5′-ACGUGACACGUUCGGAGAATT-3′ | |
YY1BS1 | Forward: 5′-GGGGAGGTGTGGGGAGG-3′ |
Reverse: 5′-AGGTGGCTAGGCGAGTTCATATG-3′ | |
YY1BS1 | Forward: 5′-GCTTGGGTTGCTGGTG GTAG-3′ |
Reverse: 5′-CCAGACTCTCAGC TTAGTAAATTCACG-3′ |
