Table 1 Coefficient of variation (CV) and mutation rate within the primer binding regions for the ddPCR assay on megaTAL treated samples.

From: Digital detection of endonuclease mediated gene disruption in the HIV provirus

293T pDHIV3 transfected cell treatments

ddPCR CV (%)

BR-Indels

A

B

6.5 megaTAL

5.2

6.5

2.3

6.5 megaTAL + Trex2

3.2

2.3

2.8

7.5 megaTAL

3.5

8.8

1.5

7.5 megaTAL + Trex2

5.9

1.6

2.0

  1. A is intraassay and B is interassay variability based on two separate runs of 3 reactions per run for each sample. BR-Indels (binding region insertion-deletions) are mutations that affect either of the two primer binding regions or reference probe binding region in sequences that have a deletion or insertion in the target binding site, representing target site mutations identified by NGS that would not be detected by ddPCR.
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