Figure 1
From: Comparative three-dimensional connectome map of motor cortical projections in the mouse brain
Targeting of layer- and subdomain-specific motor cortical neurons.
(A) Scheme for viral tracer injection in Rbp4-Cre mice for layer 5 neurons and Ntsr1-Cre mice for layer 6 neurons in the MOp and MOs. (B) Representative fluorescent images of injection sites in the motor cortex of Rbp4-Cre (left) and Ntsr1-Cre (right) mice. Scale bar: 500 μm. (C) The extent of viral expressions in the motor cortex. Numbers represent the coordinates of the anterior-posterior axis from the bregma (mm). Red and Cyan represent tdTomato signals and Green and Magenta represent GFP signals. The opacity of each color indicates relative viral-expression levels, based on the data of averaged projection signals on RSTP brain (see also Supplementary Movie S1). (D) Counting of viral-infected neurons. Representative high-resolution images of virus infected motor cortical areas (left). Yellow arrows indicate the virus infected neurons. Scale bar: 50 μm. The number of virus infected cells per transfected areas (mm2) in each cortical subdomains of each transgenic line (right). There are no statistical significant differences between MOp and MOs in both Rbp4-Cre (L5) and Ntsr1-Cre (L6) lines. P = 0.147 for Rbp4-Cre (L5), n = 5; P = 0.938 for Ntsr1-Cre (L6), n = 5, paired t-test.
