Figure 7: HDAC1 mediates the exosome-induced Foxp3 expression in Th2 cells.

OVA-specific polarized Th2 cells were prepared. The treatment was denoted in the individual panels. The cell extracts were prepared and analyzed as indicated. (A) the Western blots indicate the protein levels of HDAC1 and pHDAC1 in the Th2 cells. (B) the Western blots indicate a complex of HDAC1 and PAR2 was detected in the cell extracts of Th2 cells (by IP). (C) the bars indicate the GATA3 promoter binding rate by the pHDAC1/PAR2 complexes (by ChIP). (D) the bars indicate the mRNA levels of GATA3 and Foxp3 in Th2 cell extracts (by RT-qPCR). (E) the Western blots indicate the protein levels of Foxp3 and GATA3 in the cell extracts of Th2 cells. (F) the Western blots indicate the results of HDAC1 gene silence. HDAC1-n: HDAC1-deficiency. PAR2-n: PAR2-deficiency. cshRNA: Control shRNA. The data of bars are presented as mean ± SD. *p < 0.01, compared to the group IgG (C), or the group EE/OVA (D). The data are representative of 3 independent experiments.