Figure 3
Phe affected markers of cardiac hypertrophy in H9 C2 cells and rat hearts.
(A–D) Treatment with Phe effectively increased the mRNA levels of ANP and BNP in H9C2 cells and rat hearts. Means of exposures not sharing a common letter are significantly different at P < 0.05 (Dunnett). (E,F) After exposure to a series of Phe concentrations, a significant increase of the c-Myc expression was visualized in H9C2 cells and rat hearts. Intensities of c-Myc protein bands were quantified using densitometry. Results are expressed as multiples (×folds) of optical density of target protein and the alpha-tubulin determined in the control. The mean protein expression from the control was designated as 1 in the graph. Values (means ± S.D.) are representative of data obtained in three independent experiments (n = 3). Treatments not sharing a common letter are significantly different at P < 0.05 as assessed by one-way ANOVA followed by the Dunnett’s test.
