Figure 2

In vitro proteolytic processing of modified Cry proteins by BPH gut proteases.
Processed Cry1Ab fragments were detected by western blot with a rabbit polyclonal Cry1Ab serum as the primary antibody and anti-rabbit-IgG conjugated to HRP as the secondary antibody. Cry1Ab protoxins processed by trypsin and without processing were used as positive and negative controls, respectively.