Figure 2
From: Immunoglobulin G Expression in Human Sperm and Possible Functional Significance
IgG could be detected in the neck and the tail of human sperm with immuno-electron microscopy.
Normal human sperm was processed and stained with rabbit anti-human IgG antibody (γ chain specific) and the secondary antibody to rabbit IgG was labeled with 10 nm colloidal gold. The positive stain was shown by highly electron-dense gold particles. (a) The neck region of sperm contains abundant gold particles (white arrow) showing the subcellular distribution of IgG. Nu is nucleus and Ce is centriole. (b) Lower magnification of (a). (c) The middle piece of a sperm tail. The gold particles (white arrow) depict IgG distribution. Mi is mitochondria and Ax is axoneme. (d) Lower magnification of (c). The results demonstrate that both the neck region and the middle piece of the tail of human sperm contain synthesis IgG γ chain molecule.
