Figure 2
Impact of BKM-120 on GBM cell migration and morphology.
(A) Time-lapse images of U251-GFP GBM cells in wound-healing assays performed on U251-copGFP with increasing concentrations of BKM-120 (0.5, 1 and 2 μM) over 48 h. Controls were treated with DMSO. The gap is indicated by double arrows and the migration index was measured as total area of the gap (bar = 100 μm). (B) Time course over 72 hours on nanofiber scaffolds of G9-copGFP GBM cells treated with DMSO or BKM-120 (2 μM). The wash out performed at 48 hours demonstrates recovery of cell migration after drug removal. The lower graph indicates percentage of migration index compared to controls at 72 hours (100%) (bar = 100 μm). (C) Dose-dependent effect of BKM-120 on U251 GBM cells in transwell assays after 6 hours. The images show the ImageJ generated masks used to quantify the cell migration. Cell viability assays were performed on the same cells at the same time point using PrestoBlue. (D) Cell morphology. Time course of U251-copGFP GBM cell shape changes after treatment with 2 μM BKM-120 (bar = 50 μm). The graph indicates the percentage of total area compared to time 0h at 6, 12 and 24 hours (100%) (bar = 100 μm). One-way ANOVA was used for statistical analysis (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
