Figure 12
GSK-3β regulates Nrf2/ARE-driven genes in the cerebral cortex of rats after middle cerebral artery occlusion-reperfusion (MCAO/R).
Protein and RNA were collected after MCAO for 1 h and reperfusion for 6 h. (A) Western blot analysis of HO-1 and NQO1. (B,C) Representative ratios of HO-1 and NQO1 to β-actin. (D,E) Representative HO-1 and NQO1 mRNA levels analyzed by quantitative RT-PCR. Expression levels of HO-1 and NQO1 significantly increased in the siRNA and inhibitor groups. Results from quantitative RT-PCR were consistent with those from western blot analysis. Bars represent mean ± SEM (n = 4–6). #p < 0.05 vs. MCAO/R, ##P < 0.01 vs. MCAO/R.
