Figure 6
GSK-3β regulates Nrf2/ARE-driven genes in neurons after oxygen and glucose deprivation/reoxygenation (OGD/R).
Protein and RNA were collected after OGD for 1.5 h and reoxygenation for 1 h. (A) Western blot analysis of HO-1 and NQO1. (B,C) Representative ratios of HO-1 and NQO1 to β-actin. (D,E) Representative HO-1 and NQO1 mRNA levels analyzed by quantitative RT-PCR. The expression levels of HO-1 and NQO1 significantly increased in the siRNA (GSK-3β siRNA) + OGD/R and inhibitors + OGD/R groups. Reverse results were obtained in the GSK-3β + OGD/R group. Results from quantitative RT-PCR were consistent with those from western blot analysis. Bars represent mean ± SEM (n = 4–6). #p < 0.05 vs. OGD/R, ##p < 0.01 vs. OGD/R.
