Figure 8
GSK-3β regulation of Nrf2 in the cerebral cortex of rats in normal conditions.
(A) Western blot analyses of GSK-3β, p-GSK-3β (tyr216), Nrf2 and nuclear Nrf2. (B–E) Representative ratios of GSK-3β, p-GSK-3β (tyr216), Nrf2 and nuclear Nrf2 to β-actin. GSK-3β and p-GSK-3β (tyr216) expression significantly decreased in the siRNA (GSK-3β siRNA) and inhibitor [SB(SB216763) and LiCl] groups compared with the normal group. There was no significant difference in the expression levels of Nrf2 and nuclear Nrf2 in the siRNA and inhibitor groups compared with the normal group. Bars represent mean ± SEM (n = 4–6). *p < 0.05 vs. Normal, **p < 0.01 vs. Normal.
