Figure 1 | Scientific Reports

Figure 1

From: Corticotropin releasing factor excites neurons of posterior hypothalamic nucleus to produce tachycardia in rats

Figure 1

Quantitative real-time RT-PCR and immunofluorescence results showing that CRFR1 and CRFR2 were expressed in the rat PH.

(A) Sagittal view of the rat brain (Paxinos and Watson, 2007), localizing the PH between −3.36 and −4.36 mm from the bregma. (B) Bar graphs showing relative expression of CRFR1 and CRFR2 in the rat PH. (C1–C3) CRFR1 was present in the rat PH. (D1,D2) CRFR2 was present in the PH. (E1,E2) CRFR1 and CRFR2 were co-localized in the same PH neurons. Scale bars: (C1), (D1) and (E1), 550 μm; (C2), (D2) and (E2), 160 μm; (C3), (D3) and (E3), 20 μm. 3V, 3th ventricle; mt, mammillothalamic tract; PH, posterior hypothalamic nucleus.

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