Figure 2
From: Modulation of IL-1β reprogrammes the tumor microenvironment to interrupt oral carcinogenesis
Silencing IL-1β inhibits OSCC growth in vitro and in vivo.
(A) IL-1β protein expression levels in Cal27 cells after transfected with IL-1β shRNA (LV-shIL-1β) or LV-shNC. (B) After transfected with LV-shIL-1β or LV-shNC, Cal27 cells cell viability was measured every 24 h. (*P < 0.05 vs. cells transfected with LV-shNC) (C,D) After transfected with LV-shIL-1β or LV-shNC, Cal27 were subcutaneously injected into the flank region of nude mice. Tumor volumes were measured once every 4 days. 20 days later the mice were sacrificed and the tumors were removed and weighed. Tumor volumes (C) and weights (D) are presented as the mean ± SD. (*P < 0.05 vs. control). (E) Representative images of xenograft tumors obtained after the 20th day from Cal27 inoculation. (F) Microarray analysis of Cal27 cells transfected with LV-shIL-1β or LV-shNC. ShIL-1-1, 2, 3 from LV-shIL-1β group, NC-1, 2, 3 from LV-shNC group. (G) A total of 125 significantly changed central node genes were selected from above Cal27 microarray data to construct a multicenter interaction network. Upregulated node genes are marked in red and downregulated node genes are marked in blue.
