Figure 2

Optimization of the lectin microarray based strategy for sperm surface glycan analysis.
(a,b) Effect of fixation on sperm-lectin binding; the average SNRs of the total lectins (a) and the five representative lectins (b) showing non-significant differences between the fixed sperm and the fresh ejaculated sperm; (c–e) the optimization of sperm concentration for lectin microarray analysis from 0.5–10 × 106 sperm/block; the average SNRs of the total lectins (c) and the five representative lectins (d,e) depending on the sperm number.