Figure 3
Crystal structure of galectin-4N-lactose complex.
(A) Simulated annealing omit map of galectin-4N binding site with bound lactose. Galectin-4N binding site residues (green carbons) with the bound lactose (yellow carbon) depicted in stick representation. The 2|Fo| – |Fc|αcalc map is illustrated in blue mesh at a 1.0 σ contour level and the |Fo| – |Fc|αcalc map is illustrated in red mesh at a 3.0 σ contour level. The omit map was created by the “composite omit map” task of Phenix89. (B) Binding site interactions of galectin-4N and lactose. Galectin-4N binding site depicted in green cartoon representation with binding site residues (carbon (green)) and bound lactose (carbon (yellow)) represented as sticks. Hydrogen bonds are illustrated as dashes.
