Figure 6 | Scientific Reports

Figure 6

From: A novel nitro-dexamethasone inhibits agr system activity and improves therapeutic effects in MRSA sepsis models without antibiotics

Figure 6

Treatment of MRSA with ND8008 inhibits the activation of agr and RNAIII.

In vitro expression of icaA (a), agrA (b) and RNAIII (c) in MRSA studied at 3, 8 and 24 h of growth with 50 μM ND8008, Dex, ISMN or Dex + ISMN treatment. Data were obtained by RT-PCR and the relative transcript levels of agrA, icaA and RNAIII represent the mean (±standard deviations) of at least two biological replicates. The relative magnitude of the gene expression level was defined as the copy number of cDNA of each gene in the MRSA cells normalized by the copy number of cDNA of the corresponding gene in cells of the control group. SDS-PAGE analysis of MRSA surface proteins (c) and secretory proteins (d) after treatment with 50 μM ND8008, Dex or ISMN. (e) Immunoblot for alpha hemolysin in the supernatants from the MRSA cells with 50 μM ISMN, Dex or 50 μM, 5 μM, 0.5 μM or 0.05 μM ND8008. **P < 0.01 versus the control (Ctrl). *P < 0.05 versus the control. The same volume of ethanol was added to the controls in place of the drugs.

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