Figure 7

ND8008 had a potent anti-inflammatory activity in vivo but did not affect the bacterial load in organs.
(a–c) Effects of ND8008, Dex, ISMN or Dex + ISMN treatment on MRSA bacterial clearance in the plasma (a), spleen (b) and kidney homogenates (c) at 3, 6 and 12 h following inoculation. (d–f) Effect of ND8008, Dex, ISMN or Dex + ISMN treatment on cytokine levels during MRSA sepsis, respectively, for IL-1β (d), IL-6 (e) and TNF-α (f) levels in plasma obtained at 3, 6 and 12 h after bacterial inoculation. Data are shown as the mean ± SEM of 6 animals per group. *P < 0.05 versus the control. ***P < 0.001 versus the control. (g) Hematoxylin & eosin staining of kidneys from mice with ND8008, Dex, ISMN or Dex + ISMN treatment. Three days after inoculation, the kidneys were harvested and stained. Representative histopathological sections from 5 mice per group are shown (magnification = 200× (upper panels) and 400× (lower panels).