Figure 3

Proliferation of neural stem cells is enhanced in hTM-DTA^iPet1 mice.

(a) The number of BrdU⁺ cells (red) in the SGZ is dramatically increased in hTM-DTA^iPet1 mice. Hoechst counterstaining is shown in blue. (b,c) The number of MCM2⁺ (red in b) and Ki67⁺ cells (red in c) in the SGZ is significantly increased in hTM-DTA^iPet1 mice. Hoechst counterstaining is shown in blue. (d) Quantification of data in (a–c). Data of BrdU⁺ cell were compared using Mann-Whitney rank sum test (p = 0.016) and others using Student’s t-test (p = 0.002 for MCM2⁺ cells, p = 0.045 for Ki67⁺ cells).(e, f) The numbers of GFAP⁺/Sox2⁺ cells (QNPs, arrows) and GFAP⁻/Sox2⁺ cells (ANPs, arrowheads) are both significantly increased in the SGZ of hTM-DTA^iPet1 mice relative to controls. Data were compared using Student’s t-test (p = 0.004 for GFAP⁺/Sox2⁺ cells, p = 0.013 for GFAP^-/Sox2⁺ cells). All data are presented as mean ± s.e.m. *p < 0.05; **p < 0.01. Scale bars represent 100 μm (a), 25 μm (b,c) and 50 μm (e).