Figure 5

MAIT cells induce the cell cycle arrest of HCT116 in a cell-cell contact-dependent manner.

MAIT cells were purified from five healthy controls and stimulated with, or without, PMA/ionomycin for 48 h. The unstimulated or activated MAIT cells were co-cultured with GFP⁺ HCT116 cells in a cell-cell contact or non-contact manner. The MAIT cells or HCT116 cells in medium alone served as the controls, as illustrated in (a). Subsequently, the levels of TNF-α, IFN-γ and IL-17 in the supernatants of cultured cells were determined by CBA and the cell cycle status of HCT116 cells was examined by flow cytometry. Data are representative histograms of cell cycling or expressed as the mean values or the mean ± SD of each group from three separate experiments. (b) The levels of cytokines. The dashy lines indicate the detection limits for these cytokines by the CBA. (c) Analysis of cell cycling in HCT116 cells following co-culture with MAIT cells with or without anti-MR1 (α-MR1). (d) Dose curves of MAIT cell activity.