Figure 3
From: Role of autophagy in cadmium-induced apoptosis of primary rat osteoblasts
Autophagy regulated Cd-induced apoptosis in OBs.
OBs were treated with Cd (2 μM) alone, or pretreated with RAP (100 nM) for 1 h or with CQ (5 μM) for 30 min followed by Cd (2 μM) for a further 3 h. (A) Western blot analyses of LC3 expression in treated cells. Blots for LC3-II (B) in OBs were semi-quantified using Image LabTM software. (C) The population of apoptotic cell was calculated. Normalized cell index represents cell viability determined by RTCA in OBs after treatment. (E) Apoptosis was determined by flow cytometry for Annexin-V-FITC and propidium iodide (PI) dual labeling. Cells in the Q2 and Q4 quadrants represent apoptotic cells. The mean is present in the Q2 quadrant. (D) We calculated the population of apoptotic cell. Data are expressed as mean ± SD (n = 3) relative to control.**P < 0.01 in comparison to the control by one-way ANOVA. #P < 0.05 in comparison to the Cd treatment by one-way ANOVA. ##P < 0.01 in comparison to the Cd treatment by one-way ANOVA.
