Figure 4
From: Role of autophagy in cadmium-induced apoptosis of primary rat osteoblasts
Beclin 1 siRNA inhibits autophagosome formation and enhances the cytotoxicity induced by Cd in OBs.
OBs were treated with 2 μM Cd for an additional 3 h after transfection with random siRNA or beclin 1 siRNA for 24 h. (A) Immunoblotting for beclin 1 and LC3 using lysates from treated cells. Blots for LC3-II (C) and Beclin 1 (D) in OBs were semi-quantified using Image LabTM software. (B) Normalized cell index representing cell viability was determined by RTCA in OBs after treatment. (E) Apoptosis was determined by flow cytometry with Annexin V/PI staining. (F) We calculated the population of apoptotic cell. Data are expressed as mean ± SD (n = 3) relative to control.**P < 0.01 in comparison to the control by one-way ANOVA. #P < 0.05 in comparison to the Cd treatment by one-way ANOVA. ##P < 0.01 in comparison to the Cd treatment by one-way ANOVA.
