Figure 1

EVTs redirect the differentiation of DC to a CD1a⁻CD14⁺ phenotype.

(A) CD14⁺ monocytes were cultured for 5 d with GM-CSF and IL-4 in the absence (imDC) or presence of seminal plasma (H-imDC). The HTR8:CD14⁺ cells ratio of 1:10 was used in coculture system. Cells obtained from day3 and day5 of culture were analyzed by flow cytometry. The expression of CD1a and CD14 was displayed gated on CD11c⁺ cells. Dot plots from a representative experiment (n = 8) are shown. (B)The percentages of CD1a⁺ and CD14⁺ cells in DCs in HTR-8/ monocytes cocultures at ratios ranging from 1:2 to 1:50 were assessed by flow cytometry at day 5. Experiments were performed using monocyte from 8 distinct donors. (C) The phenotype of imDC and H-imDC (the ratio of HTR-8:monocyte is 1:10, day5) were analyzed by flow cytometry. Representative histograms from at least 5 individual experiments are shown. (isotype control Ab: gray filled histogram; indicated mAbs: open histogram) (D,E) The summarized results of surface markers expression are shown as the percentage (D) and the mean fluorescence intensity (E). The bar graphs represent mean ± EM of five individual experiments (*P < 0.05; **P < 0.01)