Figure 5 | Scientific Reports

Figure 5

From: Human monocytes undergo functional re-programming during differentiation to dendritic cell mediated by human extravillous trophoblasts

Figure 5

High concentration of MCP-1 and M-CSF in EVT-DC coculture system.

CD14+ monocytes were cultured with GM-CSF and IL-4 in the absence (DC)or presence of HTR8(H-DC), or separated with HTR8 in a transwell system(trans-DC). The cell-free supernatants of different cultures were collected at day 5 of differentiation process. The concentration of MCP-1, IL-6, IL-10, G-CSF and IFN-γ were analyzed by the Bio-Plex Protein Array system. The production of M-CSF was examined by ELISA. Data are the mea ± SEM from 3 independent experiments. *p < 0.05, ***p < 0.001.

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