Figure 2
Focal adhesion changes in human MSCs experiencing fluid shear stress on various topographies.
(A) Representative images of the focal adhesions of human MSCs, on planar PDMS, gratings and wells, stained for focal adhesion kinase (FAK) in static conditions and after shear stress application. Scale bar: 5 μm. White arrows represent the direction of the gratings. (B) Graph showing the fold change in the total number of focal adhesions formed in the cells exposed to fluid shear stress with respect to those in static conditions, on different topographies, **p < 0.01, *p < 0.05. 90 cells were analysed in three independent experiments. (C) Graph showing the fold change in focal adhesion area in human MSCs under shear stress with respect to those under static conditions on different topographies, **p < 0.01. 75 cells were analysed in three independent experiments. (D) Rose plot of the focal adhesion angles in human MSCs with respect to the direction of flow, with or without shear stress, on different topographies.
