Figure 2 | Scientific Reports

Figure 2

From: Promotion of mitotic catastrophe via activation of PTEN by paclitaxel with supplement of mulberry water extract in bladder cancer cells

Figure 2

Paclitaxel combined with MWE induced multinucleation and affected mitotic catastrophe-related protein expression levels in TSGH 8301 cells.

(a) TSGH 8301 cells were subjected to HE staining at 48 hr after the indicated treatments (left panel). The arrows point to the cells with multinucleation. Right panel, quantitation of the number of multinucleated cells/ 100 cells. (b) Nuclear extracts were prepared to assess Aurora A and Plk1. (c) Cytoplasmic lysates were prepared from TSGH 8301 cells treated with paclitaxel, MWE or both together at 24 and 48 hr and subjected to Western blotting analysis for the levels of Rho A and Cep55. The numbers under each blot are the intensity of each band relative to that of the control (not treated) or paclitaxel alone. The relative protein amounts were quantified and the results are normalized to that of either β-actin (cytoplasm) or Lamin A/C (nucleus). Arrow head indicated the band used for quantitation. The results shown are representative of three independent experiments with similar results.

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